propidium iodide pi staining  (Fisher Scientific)

Journal: Scientific Reports

Article Title: The combined inhibitory effect of butaselen and decitabine against lung cancer cells

doi: 10.1038/s41598-026-45054-7

Figure Lengend Snippet: BS combined with DAC showed enhanced effects in inhibiting the survival of lung cancer cells. A549 and H1299 cells were treated with BS, DAC, or BS + DAC (3:1 for A549 [BS-5 μM, DAC-1.67 μM] and 2:1 for H1299 [BS-5 μM, DAC-2.5 μM]) for 48 h, followed by Annexin V-PI staining. The results of total apoptosis (including both early and late apoptotic populations) are shown as mean ± SD. * P < 0.05, *** P < 0.001, **** P < 0.0001, compared with each indicated group by one-way ANOVA followed by Tukey’s post hoc test analysis ( n = 3).

Article Snippet: The cells were collected, and the apoptosis assay was conducted using the Annexin V-FITC/ propidium iodide (PI) Dual Staining Kit (KeyGEN) as instructed by the manufacturer and described previously .

Techniques: Staining